Journal:

Article Title: Mutations within the Programmed Cell Death 10 Gene Cause Cerebral Cavernous Malformations

doi:

Figure Lengend Snippet: PDCD10 northern blot analysis. Human adult MTN (Human 12-lane MTN Blot [Clontech]) was hybridized with a PDCD10 (upper panel) and β-actin (lower panel) human cDNA probe. Lane 1, brain; lane 2, heart; lane 3, skeletal muscle; lane 4, colon; lane 5, thymus; lane 6, spleen; lane 7, kidney; lane 8, liver; lane 9, small intestine; lane 10, placenta; lane 11, lung; and lane 12, peripheral blood leukocytes.

Article Snippet: One hundred nanograms of the full-length cDNA fragment were radiolabeled with [ 32 P]-dCTP and were used to hybridize a human adult MTN blot (Human 12-lane MTN Blot [Clontech]) in ExpressHyb solution (Clontech) at 1–2 × 10 6 cpm/ml, in accordance with manufacturer instructions.

Techniques: Northern Blot

Journal:

Article Title: Protein 4.1 R-135 Interacts with a Novel Centrosomal Protein (CPAP) Which Is Associated with the ?-Tubulin Complex

doi:

Figure Lengend Snippet: 4.1R interacts with CPAP in a yeast two-hybrid system. The CPAP (Q1) clone was first isolated by a yeast two-hybrid screen from a human lymphocyte cDNA library using the head domain (residues 1 to 209) of 4.1R-135 as bait (4.1R-HD). (A) Mapping the region of 4.1R-135 that interacts with CPAP. The constructs containing various portions of 4.1R-135 fused in-frame to the Gal4 DNA-binding domain were cotransformed with a Q1-ACT2 clone that expressed CPAP (residues 897 to 1338) fused to the activation domain of Gal4. The expression (+) or nonexpression (−) of the lacZ reporter gene using a colony-lift assay is shown. The column on the right represents the liquid assay for β-galactosidase (β-gal) activity using ONPG as a substrate. (B) Schematic drawing of the overlapping CPAP cDNA clones that span the entire coding region of CPAP.

Article Snippet: The initial CPAP cDNA clone (Q1) isolated by yeast two-hybrid screen was used as a probe to screen a human testis cDNA library (Clontech).

Techniques: Isolation, Two Hybrid Screening, cDNA Library Assay, Construct, Binding Assay, Activation Assay, Expressing, Activity Assay, Clone Assay

Journal:

Article Title: Protein 4.1 R-135 Interacts with a Novel Centrosomal Protein (CPAP) Which Is Associated with the ?-Tubulin Complex

doi:

Figure Lengend Snippet: Expression of CPAP mRNA. (Right) Blotted filters (Clontech) with 2 μg of polyadenylated RNA from various human tissues were hybridized with a 32P-labeled human CPAP cDNA probe (nt 2899 to 3423). The same blot was stripped and reprobed with β-actin to quantify RNA loading. (Left) Total RNA (20 μg) extracted from SiHa and Molt4 cells was blotted on a nylon membrane and hybridized with a 32P-labeled CPAP cDNA probe described above. Ethidium bromide staining of the 18S and 28S rRNA was used as a quantitative control. The exposure time for the blots that hybridized with the CPAP cDNA probe was 18 h. Sizes are shown in kilobases.

Article Snippet: The initial CPAP cDNA clone (Q1) isolated by yeast two-hybrid screen was used as a probe to screen a human testis cDNA library (Clontech).

Techniques: Expressing, Labeling, Staining

Journal:

Article Title: Envelope Glycoprotein Cytoplasmic Domains from Diverse Lentiviruses Interact with the Prenylated Rab Acceptor

doi: 10.1128/JVI.76.1.327-337.2002

Figure Lengend Snippet: Comparison of relative lengths of envelope glycoprotein cytoplasmic domain sequences from 10 different retroviruses. Scale is in numbers of amino acids. HTLV-1, human T-cell lymphotropic virus type 1; MPMV, Mason-Pfizer monkey virus; MoMLV, Moloney murine leukemia virus.

Article Snippet: A yeast two-hybrid screen for cellular binding partners to the SIV gp41 CD was performed using the yeast two-hybrid system 3 and a phytohemagglutinin (PHA)-activated human T-cell cDNA library (Clontech, Palo Alto, Calif.) ( 15 ).

Techniques: